Crystallographer Job Description Sample
Research Lab Specialist Senior
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Amy C. Rice
Human Resources Generalist
University of Michigan
930 North University
Ann Arbor, MI 48109-1055.
Conduct research on essential metabolic proteins. Carry out studies to understand the role of key higher eukaryotic proteins linked to the vitamin B-12, homocysteine, and folate metabolisms.
Design and perform experiments towards completion of the specific research aims on laboratory ongoing projects. These experiments would include expression and purification of proteins and metalloproteins in a variety of cell types, and their crystallographic and enzymology characterization. Analyze and interpret data derived from experiments.
Prepare manuscripts for publication in peer-reviewed journals based on experimental data generated from project. Prepare technical reports and research summaries. Develop new laboratory protocols.
Maintain laboratory records, inventories, and logbooks. Provide quality control, troubleshooting, and maintenance of specialized reagents and equipment.
Doctor of Philosophy (Ph.D.) degree in Biochemistry or Protein and Cellular Engineering and 60 months experience as a Biochemist. Position also requires:
Experience must include: 1) 60 months experience handling metalloproteins; 2) 60 months experience expressing and purifying proteins in E. Coli, insect, and mammalian cells; 3) 48 months experience performing crystallographic experiments; 4) 48 months experience developing and performing enzymology assays; and 5) 48 months experience handling and synthesizing complex vitamin B-12 analogues.Any experience requirements may be met concurrently during the same time period.
40 hours per week; 8:00 a.m. – 5:00 p.m.; $56,373 per year; Ann Arbor, Michigan.
This notice is being provided because the University of Michigan is filing an Application for Permanent Employment Certification for the above position. Any person may provide documentary evidence bearing on the Application for Permanent Employment Certification to the Certifying Officer, United States Department of Labor, Employment and Training Administration, Office of Foreign Labor Certification, Atlanta Processing Center, Harris Tower, 233 Peachtree Street, Suite 410, Atlanta, Georgia 30303.
The College of Literature, Science, and the Arts seeks to recruit and retain a diverse workforce as a reflection of our commitments to serve the diverse people of Michigan, fulfill the College's Guiding Principles, and sustain the excellence of LSA.
To learn more about diversity, equity, and inclusion visit http://lsa.umich.edu/lsa/about/diversity--equity-and-inclusion.html
Head Of Computational Chemistry (San Diego Research Site)
We are seeking a talented scientific leader with expertise in the area of protein modeling and cheminformatics to lead Vertex's computational chemistry group at our early discovery research site in San Diego, California. You will be responsible for impacting all aspects of computational protein structure-function studies and small-molecule medicinal chemistry designs at the San Diego site and will collaborate across functions to impact the San Diego research portfolio. You will effectively communicate to our global computational chemistry group and San Diego research leadership team, having an impact on both Research and Development, which will be a key component to success in this role.
You will have experience in structure analysis in the context of understanding dynamic motion and lead optimization and will interface between our protein sciences, medicinal chemistry and computational chemistry groups. Ultimately, the successful candidate will ensure effective interdepartmental and intradepartmental communication of standard methodologies of all in-silico methods/applications to drug discovery.
Guide and provide hands-on protein and small-molecule computational efforts at the San Diego, California drug discovery site to maximize the impact of computational efforts on projects in collaboration with researchers at the site
Serve as a computational science representative on cross site or external collaborations
Help to set strategy and manage research programs from ideation to go/ no go decision points
Work with the internal methods development group to develop and deploy relevant software tools to drug discovery teams; partner with external collaborators where internal gaps exist
A Ph.D. (or equivalent) in computational chemistry or a related discipline
10+ years of pharmaceutical or biotech experience with a consistent track record of protein structure-function and small-molecule design contributions to drug discovery projects
Experience in applying molecular mechanics and dynamics methods to protein structure, ligand and fragment-based drug discovery using cryoEM and/or crystallographic derived data is an asset, particularly experience with integral membrane proteins and ion channels.
Familiarity with large scale data mining and data analysis particularly in the areas of ADME(t) and chemical reactions is expected
Excellent oral and written communication skills
Associate Scientist Structural Biology
The Associate Scientist is responsible for supporting crystallization, with a focus on proteins, protein-protein complexes and protein-ligand complexes. The Associate Scientist is also responsible for develop affinity reagents to support chaperone-assisted crystallization. This individual works collaboratively and effectively with other biologists, protein chemists, crystallographers and biophysicists in determining methodologies and strategies that best support Takeda's internal and external drug discovery programs.
Use automated platforms to grow crystals for the determination of three-dimensional structures of proteins and protein/ligand co-complexes.
Perform crystal screening and optimization experiments across a number of platforms utilizing multiple methods. Interpret crystal screening and optimization data for improvement of crystal growth. Perform crystal soaking experiments. Design and perform experiments to determine cryo conditions for freezing crystals. Perform micro, streak, and macro seeding techniques, including manipulating seed crystals.
Responsible for crystallization for multiple projects and contribute to SBDD/FBDD.
Maintain crystallization and crystallography lab space, infrastructure and inventories.
Coordinate and oversee the shipping of cryo-storage vessels to synchrotrons.
Characterize proteins by spectrophotometric and chromatographic methods and improve chances of success in crystallization by modification and/or extra purification.
Design and construct and Fab libraries for phage display
Using phage display, identify and deliver high quality affinity reagents to enable chaperone assisted crystallography
EDUCATION, BEHAVIOURAL COMPETENCIES AND SKILLS:
BS degree with plus 8 years of industry experience in Structural Biology, Biochemistry, Molecular Biology, or
MS degree with plus 5 years of industry experience in Structural Biology, Biochemistry, Molecular Biology
Extensive experience in the design and execution of protein crystallization experiments, including crystal seeding, hit optimization, harvesting, cryoprotection and crystal freezing for proteins, protein-protein complexes and protein-ligand complexes
Experienced in preparation of protein samples, including buffer exchange, ligand co-concentration, limited protein purification.
Experienced with fragment based drug discovery techniques.
Deep experience utilizing automated platforms for executing crystallization experiments
Affinity reagent generation
Sound knowledge and skills in designing and executing phage (or other) display discovery campaigns in an industry setting. Troubleshoot and iterate phage discovery processes to deliver high quality affinity reagents for difficult-to-crystallize targets
Experienced in developing plate-based screening assays to identify and rank antibody leads
Strong background in molecular biology and protein chemistry – cloning, vector design, mutagenesis, protein purification and characterization.
Experienced in the design and construction of antibody display libraries.
Experience with flow cytometry.
Ability to independently design and execute experiments.
Ability to work in a fast paced, data-driven and scientifically rigorous environment.
Demonstrated ability to work successfully in a cross-functional and highly collaborative team environment.
Effective verbal and written communication skills
Excellent organizational and time management skills
Thoroughly document experiments and communicate results within and across departments and project teams
Self-motivated, and work well both independently and as part of a team.
Use hands on a regular basis to handle small laboratory equipment, including multi-channel pipettors, for liquid transfers to and from 96- and 384-well plastic assay plates
Able to lift or move up to 10 pounds on a regular basis in the course of daily work
Exposure to hazardous/toxic/dangerous chemicals
- Less than or equal to 5% travel
San Diego, CA
Crystallography Postdoctoral Scholar
Berkeley Lab's Molecular Biophysics & Integrated Bioimaging Division has an opening for a Crystallography Postdoctoral Scholar. A postdoctoral position is immediately available to study the structure and function of macromolecular complexes and enzymes using biochemical, X-ray crystallographic and electron cryo-microscopy (cryo-EM) methods. This position will be part of the Structural Biology group of the Technology Division at the Joint BioEnergy Institute (JBEI) in Emeryville, California. The biological systems to be studied are:
Glycosyl transferases responsible for the synthesis of hemicelluloses in plants
Novel glycosyl hydrolases that are able to breakdown cellulosic material for biofuels production
Enzymes involved in lignin biosynthesis, degradation and valorization
Secondary metabolite and natural product enzymes
The successful candidate will have experience characterizing enzymes using biochemical techniques, and using crystallographic methods. Familiarity with cryo-EM methods would be helpful, but not essential. Robotic hardware for performing crystallization trials and imaging results are available. Crystals will be characterized and data collected using the beamline resources of the Berkeley Center for Structural Biology at the Advanced Light Source. For appropriate systems cryo-EM data will be obtained using in-house microscopes, the Bay Area Center for Cryo-EM, and other national user facilities.
What You Will Do:
Biochemical characterization of enzymes using standard techniques.
Using robotic hardware to perform screens for crystallization conditions.
Optimization of crystallization conditions.
Biophysical characterization of protein samples using standard techniques, such as dynamic light scattering.
Characterization of crystals using the Berkeley Center for Structural Biology beamlines at the Advanced Light Source.
Collection and analysis of diffraction data, model building and structure refinement.
Characterization of samples using cryo-EM methods when samples permit.
Extensive email and verbal interaction with other researchers.
Additional Responsibilities as needed:
Use of molecular biology methods to prepare expression constructs
Protein expression and purification.
Use of small angle X-ray scattering methods to analyze macromolecular complexes in solution.
What is Required:
Ph.D. or equivalent in a scientific discipline, preferably structural biology, biology, or chemistry.
Demonstrated experience of scientific research using biochemistry techniques.
Demonstrated experience of scientific research using crystallographic methods.
Experience with macromolecular crystallization techniques.
Solid interpersonal skills and the ability to work in a team environment are critical.
Ability to communicate with a broad range of researchers.
Additional Desired Qualifications:
Experience with molecular biology and protein expression methods.
Experience with the use of small angle scattering methods.
Familiarity with robotic hardware for crystal growth.
Familiarity with cryo-EM methods.
The posting shall remain open until the position is filled.
This is a full time, 2 years, postdoctoral appointment with the possibility of renewal based upon satisfactory job performance, continuing availability of funds and ongoing operational needs. You must have less than 3 years paid postdoctoral experience. Salary for Postdoctoral positions depends on years of experience post-degree.
Full-time, M-F, exempt (monthly paid) from overtime pay.
This position is represented by a union for collective bargaining purposes.
Salary will be predetermined based on postdoctoral step rates.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at Joint BioEnergy Institute (JBEI) — Bldg. 978, 5885 Hollis St., 4th floor, Emeryville, CA.
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with the Pay Transparency Nondiscrimination Provision under 41 CFR 60-1.4. Click here to view the poster and supplement: "Equal Employment Opportunity is the Law."
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